연구성과
Through various studies,
we verified that HT042 improves height growth rate.
How does height grow?
② The synthesized growth factor combines with growth protein and
③ moves to the growth plate at the tip of the bone.
④ When growth factors act on the growth plate,
⑤ increase the number and size of chondrocytes in the growth plate, resulting in longer bones and taller bones.
In summary, growth hormone increases growth factors and growth proteins, which eventually makes you taller.
How can HT042 help you grow taller?
As a result, taking HT042 increases the number and size of chondrocytes in the growth plate,which can help in height growth.
The herbal formula HT042 induces longitudinal bone growth in adolescent female rats
J Med Food, 2010
HT042 accelerates the growth rate of bone length in rats.
[Reported that HT042 has the effect of promoting the growth rate of bone length in rats, the effect of promoting the growth of growth plate chondrocytes, the effect of increasing the length of the growth plate proliferative part, and the effect of increasing the expression of IGF-1 and BMP-2 in growth plate]
After oral administration of HT042 to 3-week-old female SD rats for 4 days, the growth rate was measured by tetracycline calcium fluorescence labeling method. As a result, the growth rate was significantly increased to a similar extent to growth hormone.
In addition, the number of chondrocytes in the tibial growth plate and the thickness of the proliferative zone were increased, and the expression of IGF-1 and BMP-2 was increased.
Skeletal growth and IGF levels in rats after HT042 treatment
Phytother Res, 2012
HT042 accelerates the body length growth rate of rats.
[Reported that HT042 has the effect of increasing the body length of rats, the effect of increasing the length of the growth plate hypertrophy, and the effect of increasing the expression of IGF-1 and IGFBP-3 in growth plate]
After oral administration of HT042 to 8-week-old female SD rats for 3 weeks, the length from the nose to the tail was measured. As a result, the body length was significantly increased to a similar extent to growth hormone.
Also, the thickness of the hypertrophic part of the tibial growth plate was increased, and the expression of IGF-1 and IGFBP-3 was increased.
Effect of HT042, herbal formula, on longitudinal bone growth in spontaneous dwarf rats
Molecules, 2013
The growth-promoting effect of HT042 appears in the growth hormone secretion-stimulating pathway.
[Reported that HT042 acts as a pathway to promote growth hormone secretion, as HT042 does not show growth-promoting effects in spontaneous dwarfism rats]
HT042 was orally administered to male and female rats with spontaneous dwarfism which is unable to produce normal growth hormone for 10 days, and the growth rate was measured by tetracycline calcium fluorescence labeling method. Unlike normal rats, the growth-promoting effect of HT042 was not shown, so it was judged that HT042 acted as a growth hormone secretion-stimulating pathway.
Effects of Phlomis umbrosa Root on Longitudinal Bone Growth Rate in Adolescent Female Rats
Molecules, 2016
Phlomis umbrosa extract, a component of HT042, accelerates the growth rate of bone length in rats.
[Reported that Phlomis umbrosa extract has the effect of promoting the growth rate of bone length in rats, the effect of promoting the growth plate chondrocyte proliferation, the effect of increasing the expression of IGF-1 and BMP-2 in growth plate, and the secretion of IGFBP-3 in the blood]
Phlomis umbrosa extract was orally administered to 25-day-old female SD rats for 10 days, and the growth rate was measured by tetracycline calcium fluorescence labeling method. As a result, the growth rate was significantly increased in a dose-dependent manner.
In addition, the number of chondrocytes in the tibial growth plate and the thickness of the hypertrophic zone were increased, and the expression of IGF-1 and BMP-2 was increased.
As a result of measuring blood concentration at 12 hours after administration of Phlomis umbrosa extract, IGFBP-3 was significantly increased.
Astragalus Extract Mixture HT042 Improves Bone Growth, Mass, and Microarchitecture in Prepubertal Female Rats: A Microcomputed Tomographic Study
Evid Based Complement Alternat Med, 2017
HT042 improves bone mass and bone microstructure as well as bone length growth rate.
[Reported that HT042 administration has the effect of accelerating the growth rate of bone length and improving bone mass and microstructure using MicroCT, which can accurately measure 3-dimensional bone length in a living state]
After administering HT042 to 21-day-old female SD rats in combination with feed for 2 weeks, 3-dimensional bone length was measured using MicroCT. As a result, bone length was significantly increased to a similar extent to growth hormone.
In addition, the bone mass and microstructure of trabecular bone, which are indicators related to the strength and quality of growing bone, were improved.
Astragalus Extract Mixture HT042 Increases Longitudinal Bone Growth Rate by Upregulating Circulatory IGF-1 in Rats
Evid Based Complement Alternat Med, 2017
HT042 accelerates growth by increasing IGF-1 in the blood.
[Reporting that HT042 promotes the expression of IGF-1 and IGFBP-3 mRNA synthesized in the liver by growth hormone, the expression of IGF-1 protein in the blood, and the phosphorylation of JAK2/STAT5 involved in IGF-1 expression]
After oral administration of HT042 to 25-day-old female SD rats for 10 days, IGF-1 blood concentration, liver IGF-1 mRNA expression, and IGFBP-3 mRNA expression were all increased.
In addition, stimulated phosphorylation of JAK2/STAT5, which is a pathway of growth hormone stimulating IGF-1 expression.
Effects of Astragalus Extract Mixture HT042 on Height Growth in Children with Mild Short Stature: A Multicenter Randomized Controlled Trial
Phytother Res, 2018
HT042 has height growth functionality not only in animal experiments but also in double blind human application trial targeting children.
[Reported that intake of HT042 stimulated height growth compared to the control group that consumed placebo, and did not shorten the growth period as it did not affect bone age or sex hormones]
In a 24-week multicenter, randomized, double blind, human application trial targeting 140 children aged 6-8 years who were 25% shorter in height, the HT042 intake group had a significantly higher amount of height grown during 24 weeks than the placebo intake group.
In addition, it was not affect the increase in bone age or the concentration of luteinizing hormone, which are indicators that shorten the growth period.
Effects of Astragalus Extract Mixture HT042 on Circulating IGF-1 Level and Growth Hormone Axis in Rats
Children, 2021
HT042 accelerates the growth rate through the growth hormone central axis.
[Reported that HT042 increases the expression of growth-stimulating IGF-1 in the blood, pituitary GH, GHRH receptor, GHS receptor, and hypothalamic GHRH and inhibits the expression of hypothalamic GH inhibitory hormone to speed up growth]
After oral administration of HT042 to 40-day-old male SD rats, the results were measured: growth factor IGF-1 concentration in blood, liver IGF-1, IGFBP-3 mRNA expression, pituitary GH, GH-secreting GHRH receptor, and GH secretion-stimulating GHS receptor expression and hypothalamic GHRH expression were both increased, and SST hypothalamic expression, which inhibits GH secretion, was suppressed.
As a result of measuring the growth rate through the tetracycline calcium fluorescence labeling method, the growth rate was significantly increased to a similar extent to that of growth hormone, and the expression of IGF-1 and BMP-2 in the tibial growth plate was increased.
We have repeatedly verified that HT042 does not cause precocious puberty, which shortens the growth period.
Accordingly, we conducted animal and cell experiments to confirm that HT042 has no sex hormone-like action or secretion-stimulating action, and does not affect reproductive organs.
In the human application test, it was confirmed that it does not increase the secretion of sex hormone (LH) that causes secondary sexual characteristics or increase bone age.
This means that HT042 can help children grow taller without shortening their natural growth period.
For more thorough verification, we have confirmed that it is safe by conducting 7 GLP toxicity tests, which are the most demanding level for the safety evaluation of pharmaceuticals.
Skeletal growth and IGF levels in rats after HT042 treatment
Phytother Res, 2012
HT042 does not show female hormone-like action or secretion-stimulating action in female rats.
[Reported that HT042 does not promote female hormone secretion and has no similar effect cause it does not change the uterine weight of ovariectomized rats and immature rats]
According to the OECD guideline for evaluating estrogenic activity, HT042 was orally administered to 8-week-old female SD rats in which female hormones are not secreted by removing ovaries and immature female SD rats of 17 days of age before female hormones are secreted for 8 weeks and 5 days, respectively. As a result of measuring the weight of the uterus after the test, HT042 did not change the weight of the uterus compared to the control group.
Safety evaluation of Astragalus extract mixture HT042 and its constituent herbs in Sprague-Dawley rats
Phytomedicine, 2017
HT042 is a non-toxic and safe ingredient in the GLP toxicity test.
[Reported that HT042 and its constituents are all safe in an acute toxicity test conducted by a GLP institution, and HT042 is safe in a 13-week repeated toxicity test]
In order to guarantee the reliability of the toxicity test conducted for the safety evaluation of pharmaceuticals, the safety of HT042 and its constituents was tested for single-dose toxicity at the GLP institution that systematically manages the entire test process.
As a result, no specific side effects were observed at all doses. Even when the administration period was extended to 13 weeks and a repeated dose toxicity test was conducted, no special side effects were found.
Effects of Astragalus Extract Mixture HT042 on Height Growth in Children with Mild Short Stature: A Multicenter Randomized Controlled Trial
Phytother Res, 2018
HT042 has height growth functionality not only in animal experiments but also in double blind human application trial targeting children.
[Reported that intake of HT042 stimulated height growth compared to the control group that consumed placebo, and did not shorten the growth period as it did not affect bone age or sex hormones]
In a 24-week multicenter, randomized, double blind, human application trial targeting 140 children aged 6-8 years who were 25% shorter in height, the HT042 intake group had a significantly higher amount of height grown during 24 weeks than the placebo intake group.
In addition, it was not affect the increase in bone age or the concentration of luteinizing hormone, which are indicators that shorten the growth period.
Effects of Astragalus Extract Mixture HT042 on H295R Steroidogenesis and Hershberger Assay
Food Suppl Biomater Health, 2021
[Reporting that HT042 did not affect the secretion of progesterone and estradiol in the steroid synthesis assay, and did not show sex hormone activity or suppression in the gonadal hypertrophy response test]
Whether HT042 causes precocious puberty or not has been studied through human application tests and animal experiments as above, because that if HT042 induces precocious puberty, it may not help the child’s final height even if the growth rate is accelerated.
In this study, H295R adrenocortical cell line, which produces most of the enzymes required for sex hormone synthesis, was treated with HT042, but it did not affect the secretion of female hormones such as progesterone and estradiol.
In addition, HT042 did not affect blood testosterone and related organs in all groups of normal rats and orchiectomized rats.